Keratin 33A ELISA Kit

Produktnummer ABIN2043855

Das Human Keratin 33A ELISA Kit (ABIN2043855) ist ein Colorimetric ELISA Kit zur Detektion von Human Keratin 33A.

Kurzübersicht für Keratin 33A ELISA Kit (ABIN2043855)

Target: Keratin 33A (KRT33A)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails Keratin 33A ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 29 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Keratin 33A (KRT33A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 33A (KRT33A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 33A (KRT33A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 33A (KRT33A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 33A (KRT33A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Keratin 33A

Target: Keratin 33A (KRT33A)