Cytokeratin 19 ELISA Kit

Produktnummer ABIN2043853

Maus Cytokeratin 19 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus Cytokeratin 19.

Kurzübersicht für Cytokeratin 19 ELISA Kit (ABIN2043853)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails Cytokeratin 19 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 13.6 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Keratin 19 (KRT19) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 19 (KRT19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 19 (KRT19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 19 (KRT19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 19 (KRT19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))