IL-10RA ELISA Kit

Produktnummer ABIN2043797

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus IL-10RA.

Kurzübersicht für IL-10RA ELISA Kit (ABIN2043797)

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails IL-10RA ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 29 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Interleukin 10 Receptor Alpha (IL10Ra) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 Receptor Alpha (IL10Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 Receptor Alpha (IL10Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 Receptor Alpha (IL10Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 10 Receptor Alpha (IL10Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Andere Bezeichnung: Interleukin 10 Receptor alpha (IL10Ra)

Pathways: Growth Factor Binding