IRF4 ELISA Kit

Produktnummer ABIN2043783

Dieses Ratte IRF4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte IRF4 zu quantifizieren.

Kurzübersicht für IRF4 ELISA Kit (ABIN2043783)

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails IRF4 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.133 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Interferon Regulatory Factor 4 (IRF4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 4 (IRF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 4 (IRF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 4 (IRF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interferon Regulatory Factor 4 (IRF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IRF4

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Pathways: Chromatin Binding