ITIH4 ELISA Kit

Produktnummer ABIN2043777

Dieses Maus ITIH4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus ITIH4 zu quantifizieren.

Kurzübersicht für ITIH4 ELISA Kit (ABIN2043777)

Target: ITIH4 (Inter alpha Globulin Inhibitor H4 (ITIH4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81-500 ng/mL

Applikation: ELISA

Produktdetails ITIH4 ELISA Kit

Untere Nachweisgrenze: 7.81 ng/mL

Sensitivität: < 3.3 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Inter Alpha-Globulin Inhibitor H4 (ITIH4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H4 (ITIH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Inter Alpha-Globulin Inhibitor H4 (ITIH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ITIH4

Target: ITIH4 (Inter alpha Globulin Inhibitor H4 (ITIH4))

Andere Bezeichnung: Inter alpha-Globulin Inhibitor H4 (ITIH4)

Pathways: Glycosaminoglycan Metabolic Process