ITGA4 ELISA Kit

Produktnummer ABIN2043769

Maus ITGA4 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus ITGA4.

Kurzübersicht für ITGA4 ELISA Kit (ABIN2043769)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails ITGA4 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.071 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Integrin Alpha 4 (ITGa4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 4 (ITGa4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 4 (ITGa4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 4 (ITGa4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Integrin Alpha 4 (ITGa4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Pathways: Integrin Complex