IRS3 ELISA Kit

Produktnummer ABIN2043765

Das Ratte IRS3 ELISA Kit (ABIN2043765) ist ein Colorimetric ELISA Kit zur Detektion von Ratte IRS3.

Kurzübersicht für IRS3 ELISA Kit (ABIN2043765)

Target: IRS3 (Insulin Receptor Substrate 3 (IRS3))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.62-40 ng/mL

Applikation: ELISA

Produktdetails IRS3 ELISA Kit

Untere Nachweisgrenze: 0.62 ng/mL

Sensitivität: < 0.227 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Insulin Receptor Substrate 3 (IRS3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor Substrate 3 (IRS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Receptor Substrate 3 (IRS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Receptor Substrate 3 (IRS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Receptor Substrate 3 (IRS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IRS3

Target: IRS3 (Insulin Receptor Substrate 3 (IRS3))