IGFBP7 ELISA Kit

Produktnummer ABIN2043761

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Schwein IGFBP7.

Kurzübersicht für IGFBP7 ELISA Kit (ABIN2043761)

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails IGFBP7 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.135 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Insulin Like Growth Factor Binding Protein 7 (IGFBP7) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 7 (IGFBP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Like Growth Factor Binding Protein 7 (IGFBP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IGFBP7

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Andere Bezeichnung: Insulin Like Growth Factor Binding Protein 7 (IGFBP7)

Pathways: Growth Factor Binding