IGF2BP3 ELISA Kit

Produktnummer ABIN2043755

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human IGF2BP3.

Kurzübersicht für IGF2BP3 ELISA Kit (ABIN2043755)

Target: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails IGF2BP3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.055 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IGF2BP3

Target: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Andere Bezeichnung: Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3)