ISLR ELISA Kit

Produktnummer ABIN2043735

Dieses Human ISLR ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human ISLR zu quantifizieren.

Kurzübersicht für ISLR ELISA Kit (ABIN2043735)

Target: ISLR (Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails ISLR ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.065 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ISLR

Target: ISLR (Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR))