IgA ELISA Kit

Produktnummer ABIN2043727

Produktdetails IgA ELISA Kit

Target: IgA

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.93-60 ng/mL

Untere Nachweisgrenze: 0.93 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.42 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Immunoglobulin A (IgA) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin A (IgA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin A (IgA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Immunoglobulin A (IgA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IgA

Target: IgA

Andere Bezeichnung: Immunoglobulin A (IgA) (IgA Produkte)

Synonyme: MB-1 ELISA Kit, IG-alpha ELISA Kit, IGA ELISA Kit, IgA ELISA Kit, Igh-2 ELISA Kit, CD79a molecule ELISA Kit, immunoglobulin heavy constant alpha ELISA Kit, CD79A ELISA Kit, Igha ELISA Kit

Substanzklasse: Antibody