HTRA4 ELISA Kit

Produktnummer ABIN2043719

Produktdetails HTRA4 ELISA Kit

Target: HTRA4 (HtrA Serine Peptidase 4 (HTRA4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.28 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: HtrA Serine Peptidase 4 (HTRA4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HtrA Serine Peptidase 4 (HTRA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HtrA Serine Peptidase 4 (HTRA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HtrA Serine Peptidase 4 (HTRA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of HtrA Serine Peptidase 4 (HTRA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HTRA4

Target: HTRA4 (HtrA Serine Peptidase 4 (HTRA4))

Abstract: HTRA4 Produkte

Synonyme: B430206E18Rik ELISA Kit, RGD1306242 ELISA Kit, HtrA serine peptidase 4 ELISA Kit, HTRA4 ELISA Kit, Htra4 ELISA Kit