HTRA1 ELISA Kit

Produktnummer ABIN2043717

Dieses Human HTRA1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human HTRA1 zu quantifizieren.

Kurzübersicht für HTRA1 ELISA Kit (ABIN2043717)

Target: HTRA1 (HtrA Serine Peptidase 1 (HTRA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails HTRA1 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 14.3 pg/mL

Bestandteile: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: The Human HtrA Serine Peptidase (HTRA1) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HTRA1 in human serum, plasma and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to HTRA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HTRA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTRA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HTRA1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HTRA1

Target: HTRA1 (HtrA Serine Peptidase 1 (HTRA1))

Pathways: Growth Factor Binding