HDAC4 ELISA Kit

Produktnummer ABIN2043703

Human HDAC4 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human HDAC4.

Kurzübersicht für HDAC4 ELISA Kit (ABIN2043703)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails HDAC4 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.55 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Histone Deacetylase 4 (HDAC4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 4 (HDAC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 4 (HDAC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 4 (HDAC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Histone Deacetylase 4 (HDAC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process