Nerve Growth Factor ELISA Kit

Produktnummer ABIN2043685

Human Nerve Growth Factor ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Nerve Growth Factor.

Kurzübersicht für Nerve Growth Factor ELISA Kit (ABIN2043685)

Target: Nerve Growth Factor (NGF)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12-200 pg/mL

Applikation: ELISA

Produktdetails Nerve Growth Factor ELISA Kit

Untere Nachweisgrenze: 3.12 pg/mL

Marke: BioAssay™

Sensitivität: < 0.95 pg/mL

Bestandteile: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: The Human Nerve Growth Factor (NGF) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NGF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NGF in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Pathways: Regulation of Cell Size