IgG ELISA Kit

Produktnummer ABIN2043665

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus IgG.

Kurzübersicht für IgG ELISA Kit (ABIN2043665)

Target: IgG

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails IgG ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.31 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: High Sensitivity Immunoglobulin G (IgG) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G (IgG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G (IgG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G (IgG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Immunoglobulin G (IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IgG

Target: IgG

Andere Bezeichnung: Immunoglobulin G (IgG)

Substanzklasse: Antibody