HDLBP ELISA Kit

Produktnummer ABIN2043645

Dieses Human HDLBP ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human HDLBP zu quantifizieren.

Kurzübersicht für HDLBP ELISA Kit (ABIN2043645)

Target: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails HDLBP ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.056 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: High Density Lipoprotein Binding Protein (HDLBP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Density Lipoprotein Binding Protein (HDLBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of High Density Lipoprotein Binding Protein (HDLBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HDLBP

Target: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))