HDGF ELISA Kit

Produktnummer ABIN2043637

Maus HDGF ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus HDGF.

Kurzübersicht für HDGF ELISA Kit (ABIN2043637)

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails HDGF ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 13.8 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Hepatoma Derived Growth Factor (HDGF) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatoma Derived Growth Factor (HDGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatoma Derived Growth Factor (HDGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatoma Derived Growth Factor (HDGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hepatoma Derived Growth Factor (HDGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HDGF

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Andere Bezeichnung: Hepatoma Derived Growth Factor (HDGF)

Pathways: ER-Nucleus Signaling