HS6ST1 ELISA Kit

Produktnummer ABIN2043627

Dieses Human HS6ST1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human HS6ST1 zu quantifizieren.

Kurzübersicht für HS6ST1 ELISA Kit (ABIN2043627)

Target: HS6ST1 (Heparan Sulfate 6-O-Sulfotransferase 1 (HS6ST1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails HS6ST1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.063 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Heparan Sulfate-6-O-Sulfotransferase 1 (HS6ST1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-6-O-Sulfotransferase 1 (HS6ST1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate-6-O-Sulfotransferase 1 (HS6ST1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-6-O-Sulfotransferase 1 (HS6ST1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heparan Sulfate-6-O-Sulfotransferase 1 (HS6ST1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HS6ST1

Target: HS6ST1 (Heparan Sulfate 6-O-Sulfotransferase 1 (HS6ST1))

Andere Bezeichnung: Heparan Sulfate-6-O-Sulfotransferase 1 (HS6ST1)

Pathways: Glycosaminoglycan Metabolic Process