HSPB3 ELISA Kit

Produktnummer ABIN2043619

Human HSPB3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human HSPB3.

Kurzübersicht für HSPB3 ELISA Kit (ABIN2043619)

Target: HSPB3 (Heat Shock 27kDa Protein 3 (HSPB3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails HSPB3 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.29 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Heat Shock Protein Beta 3 (HSPb3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 3 (HSPb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 3 (HSPb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 3 (HSPb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock Protein Beta 3 (HSPb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HSPB3

Target: HSPB3 (Heat Shock 27kDa Protein 3 (HSPB3))

Andere Bezeichnung: Heat Shock Protein beta 3 (HSPb3)