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HSP70 ELISA Kit

Dieses Huhn HSP70 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Huhn HSP70 zu quantifizieren.
Produktnummer ABIN2043615

Kurzübersicht für HSP70 ELISA Kit (ABIN2043615)

Target

Alle HSP70 ELISA Kits anzeigen
HSP70 (Heat Shock Protein 70 (HSP70))

Reaktivität

  • 7
  • 6
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  • 5
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  • 3
  • 3
  • 2
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Huhn

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

1.56-100 ng/mL

Applikation

ELISA
  • Untere Nachweisgrenze

    1.56 ng/mL

    Sensitivität

    < 0.58 ng/mL
  • Plattentyp

    Pre-coated

    Protokoll

    Heat Shock Protein 70 (HSP70) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70 (HSP70). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 70 (HSP70). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 70 (HSP70), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock Protein 70 (HSP70) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C/-20 °C
  • Target Alle HSP70 ELISA Kits anzeigen

    HSP70 (Heat Shock Protein 70 (HSP70))
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