HSPA1B ELISA Kit

Produktnummer ABIN2043613

Rind (Kuh) HSPA1B ELISA-Kit Colorimetric-Assay zur Quantifizierung von Rind (Kuh) HSPA1B.

Kurzübersicht für HSPA1B ELISA Kit (ABIN2043613)

Target: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails HSPA1B ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.53 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Heat Shock 70kDa Protein 1B (HSPA1B) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1B (HSPA1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock 70kDa Protein 1B (HSPA1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HSPA1B

Target: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))