HSPA12B ELISA Kit

Produktnummer ABIN2043609

Das Human HSPA12B ELISA Kit (ABIN2043609) ist ein Colorimetric ELISA Kit zur Detektion von Human HSPA12B.

Kurzübersicht für HSPA12B ELISA Kit (ABIN2043609)

Target: HSPA12B (Heat Shock 70kD Protein 12B (HSPA12B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails HSPA12B ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.128 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Heat Shock 70kDa Protein 12B (HSPA12B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 12B (HSPA12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 12B (HSPA12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 12B (HSPA12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock 70kDa Protein 12B (HSPA12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HSPA12B

Target: HSPA12B (Heat Shock 70kD Protein 12B (HSPA12B))

Andere Bezeichnung: Heat Shock 70kDa Protein 12B (HSPA12B)