GUCY1B3 ELISA Kit

Produktnummer ABIN2043607

Human GUCY1B3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human GUCY1B3.

Kurzübersicht für GUCY1B3 ELISA Kit (ABIN2043607)

Target: GUCY1B3 (Guanylate Cyclase 1, Soluble, beta 3 (GUCY1B3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails GUCY1B3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Guanylate Cyclase 1 Beta 3 (GUCY1b3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Cyclase 1 Beta 3 (GUCY1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Guanylate Cyclase 1 Beta 3 (GUCY1b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GUCY1B3

Target: GUCY1B3 (Guanylate Cyclase 1, Soluble, beta 3 (GUCY1B3))

Andere Bezeichnung: Guanylate Cyclase 1 beta 3 (GUCY1b3)

Pathways: Transition Metal Ion Homeostasis