GZMH ELISA Kit

Produktnummer ABIN2043595

Human GZMH ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human GZMH.

Kurzübersicht für GZMH ELISA Kit (ABIN2043595)

Target: GZMH (Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 pg/mL

Applikation: ELISA

Produktdetails GZMH ELISA Kit

Untere Nachweisgrenze: 1.56 pg/mL

Sensitivität: < 0.57 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Granzyme H (GZMH) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme H (GZMH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme H (GZMH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme H (GZMH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Granzyme H (GZMH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GZMH

Target: GZMH (Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH))

Andere Bezeichnung: Granzyme H (GZMH)