GM2A ELISA Kit

Produktnummer ABIN2043589

Human GM2A ELISA Kit, Colorimetric Assay für die Quantifizierung von Human GM2A.

Kurzübersicht für GM2A ELISA Kit (ABIN2043589)

Target: GM2A (GM2 Ganglioside Activator (GM2A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails GM2A ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: GM2 Ganglioside Activator (GM2A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM2 Ganglioside Activator (GM2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GM2 Ganglioside Activator (GM2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GM2 Ganglioside Activator (GM2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of GM2 Ganglioside Activator (GM2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GM2A

Target: GM2A (GM2 Ganglioside Activator (GM2A))