GP1BB ELISA Kit

Produktnummer ABIN2043583

Maus GP1BB ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus GP1BB.

Kurzübersicht für GP1BB ELISA Kit (ABIN2043583)

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails GP1BB ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Marke: BioAssay™

Sensitivität: < 14.7 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glycoprotein Ib Beta Polypeptide, Platelet (GP1Bb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GP1BB

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Andere Bezeichnung: Glycoprotein Ib beta Polypeptide, Platelet (GP1Bb)