GLRa2 ELISA Kit

Produktnummer ABIN2043579

Dieses Ratte GLRa2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte GLRa2 zu quantifizieren.

Kurzübersicht für GLRa2 ELISA Kit (ABIN2043579)

Target: GLRa2 (Glycine Receptor, alpha 2 (GLRa2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails GLRa2 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.115 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glycine Receptor Alpha 2 (GLRa2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Receptor Alpha 2 (GLRa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycine Receptor Alpha 2 (GLRa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycine Receptor Alpha 2 (GLRa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glycine Receptor Alpha 2 (GLRa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GLRa2

Target: GLRa2 (Glycine Receptor, alpha 2 (GLRa2))

Andere Bezeichnung: Glycine Receptor alpha 2 (GLRa2)

Pathways: Synaptic Membrane