GSTA4 ELISA Kit

Produktnummer ABIN2043573

Dieses Maus GSTA4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus GSTA4 zu quantifizieren.

Kurzübersicht für GSTA4 ELISA Kit (ABIN2043573)

Target: GSTA4 (Glutathione S-Transferase alpha 4 (GSTA4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails GSTA4 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.057 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glutathione S Transferase Alpha 4 (GSTa4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 4 (GSTa4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 4 (GSTa4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 4 (GSTa4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glutathione S Transferase Alpha 4 (GSTa4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GSTA4

Target: GSTA4 (Glutathione S-Transferase alpha 4 (GSTA4))