Glutathione Peroxidase 2 ELISA Kit

Produktnummer ABIN2043569

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Glutathione Peroxidase 2.

Kurzübersicht für Glutathione Peroxidase 2 ELISA Kit (ABIN2043569)

Target: Glutathione Peroxidase 2 (GPX2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails Glutathione Peroxidase 2 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.118 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glutathione Peroxidase 2, Gastrointestinal (GPX2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 2, Gastrointestinal (GPX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Peroxidase 2, Gastrointestinal (GPX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 2, Gastrointestinal (GPX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glutathione Peroxidase 2, Gastrointestinal (GPX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Glutathione Peroxidase 2

Target: Glutathione Peroxidase 2 (GPX2)

Andere Bezeichnung: Glutathione Peroxidase 2, Gastrointestinal (GPX2)

Pathways: Thyroid Hormone Synthesis