Glutaredoxin 1 ELISA Kit

Produktnummer ABIN2043567

Dieses Maus Glutaredoxin 1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus Glutaredoxin 1 zu quantifizieren.

Kurzübersicht für Glutaredoxin 1 ELISA Kit (ABIN2043567)

Target: Glutaredoxin 1 (GRX1)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails Glutaredoxin 1 ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 32 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glutaredoxin (GLRX) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaredoxin (GLRX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaredoxin (GLRX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaredoxin (GLRX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glutaredoxin (GLRX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Glutaredoxin 1

Target: Glutaredoxin 1 (GRX1)

Andere Bezeichnung: Glutaredoxin (GLRX)

Substanzklasse: Viral Protein

Pathways: Cell RedoxHomeostasis