GAD ELISA Kit

Produktnummer ABIN2043559

Maus GAD ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus GAD.

Kurzübersicht für GAD ELISA Kit (ABIN2043559)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails GAD ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.31 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glutamate Decarboxylase 1, Brain (GAD1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Decarboxylase 1, Brain (GAD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Decarboxylase 1, Brain (GAD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Decarboxylase 1, Brain (GAD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glutamate Decarboxylase 1, Brain (GAD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Andere Bezeichnung: Glutamate Decarboxylase 1, Brain (GAD1)