GLCE ELISA Kit

Produktnummer ABIN2043557

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte GLCE.

Kurzübersicht für GLCE ELISA Kit (ABIN2043557)

Target: GLCE (Glucuronic Acid Epimerase (GLCE))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12-200 ng/mL

Applikation: ELISA

Produktdetails GLCE ELISA Kit

Untere Nachweisgrenze: 3.12 ng/mL

Sensitivität: < 1.26 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glucuronic Acid Epimerase (GLCE) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucuronic Acid Epimerase (GLCE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucuronic Acid Epimerase (GLCE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucuronic Acid Epimerase (GLCE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucuronic Acid Epimerase (GLCE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GLCE

Target: GLCE (Glucuronic Acid Epimerase (GLCE))

Pathways: Glycosaminoglycan Metabolic Process