GAA ELISA Kit

Produktnummer ABIN2043555

Dieses Maus GAA ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus GAA zu quantifizieren.

Kurzübersicht für GAA ELISA Kit (ABIN2043555)

Target: GAA (Glucosidase, Alpha, Acid (GAA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails GAA ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 14.3 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glucosidase Alpha, Acid (GaA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Alpha, Acid (GaA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucosidase Alpha, Acid (GaA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucosidase Alpha, Acid (GaA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucosidase Alpha, Acid (GaA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GAA

Target: GAA (Glucosidase, Alpha, Acid (GAA))

Andere Bezeichnung: Glucosidase Alpha, Acid (GaA)

Pathways: Cellular Glucan Metabolic Process