G6PC ELISA Kit

Produktnummer ABIN2043553

Maus G6PC ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus G6PC.

Kurzübersicht für G6PC ELISA Kit (ABIN2043553)

Target: G6PC (Glucose 6-Phosphatase, Catalytic (G6PC))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails G6PC ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.52 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glucose-6-Phosphatase, Catalytic (G6PC) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose-6-Phosphatase, Catalytic (G6PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucose-6-Phosphatase, Catalytic (G6PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu G6PC

Target: G6PC (Glucose 6-Phosphatase, Catalytic (G6PC))

Andere Bezeichnung: Glucose-6-Phosphatase, Catalytic (G6PC)

Pathways: Carbohydrate Homeostasis, Cellular Glucan Metabolic Process