Glucose-6-Phosphate Dehydrogenase ELISA Kit

Produktnummer ABIN2043549

Produktdetails Glucose-6-Phosphate Dehydrogenase ELISA Kit

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Untere Nachweisgrenze: 0.31 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.137 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glucose 6 Phosphate Dehydrogenase (G6PD) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Dehydrogenase (G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucose 6 Phosphate Dehydrogenase (G6PD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: Glucose 6 Phosphate Dehydrogenase (G6PD) (G6PD Produkte)

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Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones