GLP-2 ELISA Kit

Produktnummer ABIN2043547

Schwein GLP-2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Schwein GLP-2.

Kurzübersicht für GLP-2 ELISA Kit (ABIN2043547)

Target: GLP-2 (Glucagon-like peptide 2 (GLP-2))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 123.46-10000 pg/mL

Applikation: ELISA

Produktdetails GLP-2 ELISA Kit

Untere Nachweisgrenze: 123.46 pg/mL

Sensitivität: < 43.8 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glucagon Like Peptide 2 (GLP2) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon Like Peptide 2 (GLP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucagon Like Peptide 2 (GLP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucagon Like Peptide 2 (GLP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucagon Like Peptide 2 (GLP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GLP-2

Target: GLP-2 (Glucagon-like peptide 2 (GLP-2))

Andere Bezeichnung: Glucagon Like Peptide 2 (GLP2)