GLP1R ELISA Kit

Produktnummer ABIN2043545

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human GLP1R.

Kurzübersicht für GLP1R ELISA Kit (ABIN2043545)

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails GLP1R ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.118 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glucagon Like Peptide 1 Receptor (GLP1R) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon Like Peptide 1 Receptor (GLP1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucagon Like Peptide 1 Receptor (GLP1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucagon Like Peptide 1 Receptor (GLP1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucagon Like Peptide 1 Receptor (GLP1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GLP1R

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Andere Bezeichnung: Glucagon Like Peptide 1 Receptor (GLP1R)

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, cAMP Metabolic Process, Feeding Behaviour