GFRA1 ELISA Kit

Produktnummer ABIN2043543

Dieses Human GFRA1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human GFRA1 zu quantifizieren.

Kurzübersicht für GFRA1 ELISA Kit (ABIN2043543)

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails GFRA1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.119 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GFRA1

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Andere Bezeichnung: Glial Cell Line Derived Neurotrophic Factor Receptor alpha 1 (GFRa1)