GSAP ELISA Kit

Produktnummer ABIN2043535

Ratte GSAP ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte GSAP.

Kurzübersicht für GSAP ELISA Kit (ABIN2043535)

Target: GSAP (PION) (Pigeon Homolog (PION))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails GSAP ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.113 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Gamma-Secretase Activating Protein (gSAP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Secretase Activating Protein (gSAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Secretase Activating Protein (gSAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Secretase Activating Protein (gSAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Gamma-Secretase Activating Protein (gSAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GSAP

Target: GSAP (PION) (Pigeon Homolog (PION))

Andere Bezeichnung: gamma-Secretase Activating Protein (gSAP)