FBLN3 ELISA Kit

Produktnummer ABIN2043483

Das Maus FBLN3 ELISA Kit (ABIN2043483) ist ein Colorimetric ELISA Kit zur Detektion von Maus FBLN3.

Kurzübersicht für FBLN3 ELISA Kit (ABIN2043483)

Target: FBLN3 (Fibulin 3 (FBLN3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails FBLN3 ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Sensitivität: < 6.6 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Fibulin 3 (FBLN3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 3 (FBLN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 3 (FBLN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 3 (FBLN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fibulin 3 (FBLN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu FBLN3

Target: FBLN3 (Fibulin 3 (FBLN3))

Pathways: EGFR Signaling Pathway