FCGBP ELISA Kit

Produktnummer ABIN2043447

Dieses Human FCGBP ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human FCGBP zu quantifizieren.

Kurzübersicht für FCGBP ELISA Kit (ABIN2043447)

Target: FCGBP (Fc Fragment of IgG Binding Protein (FCGBP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails FCGBP ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.116 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Fc Fragment Of IgG Binding Protein (FcgBP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fc Fragment Of IgG Binding Protein (FcgBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fc Fragment Of IgG Binding Protein (FcgBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fc Fragment Of IgG Binding Protein (FcgBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fc Fragment Of IgG Binding Protein (FcgBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu FCGBP

Target: FCGBP (Fc Fragment of IgG Binding Protein (FCGBP))