FAM135B ELISA Kit

Produktnummer ABIN2043433

Maus FAM135B ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus FAM135B.

Kurzübersicht für FAM135B ELISA Kit (ABIN2043433)

Target: FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails FAM135B ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.057 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Family With Sequence Similarity 135, Member B (FAM135B) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 135, Member B (FAM135B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Family With Sequence Similarity 135, Member B (FAM135B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 135, Member B (FAM135B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Family With Sequence Similarity 135, Member B (FAM135B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu FAM135B

Target: FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))