EIF6 ELISA Kit

Produktnummer ABIN2043419

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human EIF6.

Kurzübersicht für EIF6 ELISA Kit (ABIN2043419)

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails EIF6 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.123 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Eukaryotic Translation Initiation Factor 6 (EIF6) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 6 (EIF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 6 (EIF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 6 (EIF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Eukaryotic Translation Initiation Factor 6 (EIF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu EIF6

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly