ENGASE ELISA Kit

Produktnummer ABIN2043401

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human ENGASE.

Kurzübersicht für ENGASE ELISA Kit (ABIN2043401)

Target: ENGASE (Endo-beta-N-Acetylglucosaminidase (ENGASE))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails ENGASE ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.125 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Endo Beta-N-Acetylglucosaminidase (ENGASE) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endo Beta-N-Acetylglucosaminidase (ENGASE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endo Beta-N-Acetylglucosaminidase (ENGASE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endo Beta-N-Acetylglucosaminidase (ENGASE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Endo Beta-N-Acetylglucosaminidase (ENGASE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ENGASE

Target: ENGASE (Endo-beta-N-Acetylglucosaminidase (ENGASE))

Andere Bezeichnung: Endo beta-N-Acetylglucosaminidase (ENGASE)