ETFB ELISA Kit

Produktnummer ABIN2043393

Dieses Human ETFB ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human ETFB zu quantifizieren.

Kurzübersicht für ETFB ELISA Kit (ABIN2043393)

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails ETFB ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.112 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Electron Transfer Flavoprotein Beta Polypeptide (ETFb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Beta Polypeptide (ETFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Electron Transfer Flavoprotein Beta Polypeptide (ETFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ETFB

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Andere Bezeichnung: Electron Transfer Flavoprotein beta Polypeptide (ETFb)