EDIL3 ELISA Kit

Produktnummer ABIN2043385

Human EDIL3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human EDIL3.

Kurzübersicht für EDIL3 ELISA Kit (ABIN2043385)

Target: EDIL3 (DEL1) (EGF like repeats and discoidin domains 3 (DEL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails EDIL3 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.131 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu EDIL3

Target: EDIL3 (DEL1) (EGF like repeats and discoidin domains 3 (DEL1))

Andere Bezeichnung: EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3)