EGFL7 ELISA Kit

Produktnummer ABIN2043379

Human EGFL7 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human EGFL7.

Kurzübersicht für EGFL7 ELISA Kit (ABIN2043379)

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12-200 ng/mL

Applikation: ELISA

Produktdetails EGFL7 ELISA Kit

Untere Nachweisgrenze: 3.12 ng/mL

Sensitivität: < 1.38 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: EGF Like Domain Protein, Multiple 7 (EGFL7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 7 (EGFL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of EGF Like Domain Protein, Multiple 7 (EGFL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu EGFL7

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Andere Bezeichnung: EGF Like Domain Protein, Multiple 7 (EGFL7)