DPYS ELISA Kit

Produktnummer ABIN2043345

Dieses Human DPYS ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human DPYS zu quantifizieren.

Kurzübersicht für DPYS ELISA Kit (ABIN2043345)

Target: DPYS (Dihydropyrimidinase (DPYS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails DPYS ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.31 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Dihydropyrimidinase (DPYS) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidinase (DPYS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidinase (DPYS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidinase (DPYS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dihydropyrimidinase (DPYS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu DPYS

Target: DPYS (Dihydropyrimidinase (DPYS))