DSE ELISA Kit

Produktnummer ABIN2043329

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human DSE.

Kurzübersicht für DSE ELISA Kit (ABIN2043329)

Target: DSE (Dermatan Sulfate Epimerase (DSE))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails DSE ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.114 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Dermatan Sulfate Epimerase (DSE) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermatan Sulfate Epimerase (DSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermatan Sulfate Epimerase (DSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermatan Sulfate Epimerase (DSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dermatan Sulfate Epimerase (DSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu DSE

Target: DSE (Dermatan Sulfate Epimerase (DSE))

Pathways: Glycosaminoglycan Metabolic Process